How can I get stronger staining for my lymph node sections

I am using the same protocol and same antibodies that the literature says but still I cannot get good staining for my lymph node sections, I tried to change the fixation method and I am using now acetone instead of PFA, I also tried with acetone plus ethanol, the antibody concentration used worked in previous sections. How could I increase the signal and/or remove the noise?

It also happened to work in some sections before but not in others even I did exactly the same, why could that be if I am using the same tissue?


Watch the video: Anterior Ivocolor Stain and Glaze Technique (December 2021).