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5.5: Lichens - Biology

5.5: Lichens - Biology


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Skills to Develop

  • Explain why lichens are included in the study of microbiology
  • Describe the unique characteristics of a lichen and the role of each partner in the symbiotic relationship of a lichen
  • Describe ways in which lichens are beneficial to the environment

No one has to worry about getting sick from a lichen infection, but lichens are interesting from a microbiological perspective and they are an important component of most terrestrial ecosystems. Lichens provide opportunities for study of close relationships between unrelated microorganisms. Lichens contribute to soil production by breaking down rock, and they are early colonizers in soilless environments such as lava flows. The cyanobacteria in some lichens can fix nitrogen and act as a nitrogen source in some environments. Lichens are also important soil stabilizers in some desert environments and they are an important winter food source for caribou and reindeer. Finally, lichens produce compounds that have antibacterial effects, and further research may discover compounds that are medically useful to humans.

Characteristics

A lichen is a combination of two organisms, a green alga or cyanobacterium and an ascomycete fungus, living in a symbiotic relationship. Whereas algae normally grow only in aquatic or extremely moist environments, lichens can potentially be found on almost any surface (especially rocks) or as epiphytes (meaning that they grow on other plants).

In some ways, the symbiotic relationship between lichens and algae seems like a mutualism (a relationship in which both organisms benefit). The fungus can obtain photosynthates from the algae or cyanobacterium and the algae or cyanobacterium can grow in a drier environment than it could otherwise tolerate. However, most scientists consider this symbiotic relationship to be a controlled parasitism (a relationship in which one organism benefits and the other is harmed) because the photosynthetic organism grows less well than it would without the fungus. It is important to note that such symbiotic interactions fall along a continuum between conflict and cooperation.

Lichens are slow growing and can live for centuries. They have been used in foods and to extract chemicals as dyes or antimicrobial substances. Some are very sensitive to pollution and have been used as environmental indicators.

Lichens have a body called a thallus, an outer, tightly packed fungal layer called a cortex, and an inner, loosely packed fungal layer called a medulla (Figure (PageIndex{1})). Lichens use hyphal bundles called rhizines to attach to the substrate.


Figure (PageIndex{1}): This cross-section of a lichen thallus shows its various components. The upper cortex of fungal hyphae provides protection. Photosynthesis occurs in the algal zone. The medulla consists of fungal hyphae. The lower cortex also provides protection. The rhizines anchor the thallus to the substrate.

Lichen Diversity

Lichens are classified as fungi and the fungal partners belong to the Ascomycota and Basidiomycota. Lichens can also be grouped into types based on their morphology. There are three major types of lichens, although other types exist as well. Lichens that are tightly attached to the substrate, giving them a crusty appearance, are called crustose lichens. Those that have leaf-like lobes are foliose lichens; they may only be attached at one point in the growth form, and they also have a second cortex below the medulla. Finally, fruticose lichens have rounded structures and an overall branched appearance. Figure (PageIndex{2}) shows an example of each of the forms of lichens.

Figure (PageIndex{2}): Examples of the three types of lichens are shown here. (a) This is a crustose lichen found mostly on marine rocks, Caloplaca marina. (b) This is a foliose lichen, Flavoparmelia caperata. (c) This is a fruticose lichen, Letharia vulpina, which is sufficiently poisonous that it was once used to make arrowheads. (credit b, c: modification of work by Jason Hollinger)

Exercise (PageIndex{1})

  1. What types of organisms are found in lichens?
  2. What are the three growth forms of lichens?

Key Concepts and Summary

  • Lichens are a symbiotic association between a fungus and an algae or a cyanobacterium
  • The symbiotic association found in lichens is currently considered to be a controlled parasitism, in which the fungus benefits and the algae or cyanobacterium is harmed
  • Lichens are slow growing and can live for centuries in a variety of habitats
  • Lichens are environmentally important, helping to create soil, providing food, and acting as indicators of air pollution

Multiple Choice

You encounter a lichen with leafy structures. Which term describes this lichen?

A. crustose
B. foliose
C. fruticose
D. agarose

B

Which of the following is the term for the outer layer of a lichen?

A. the cortex
B. the medulla
C. the thallus
D. the theca

A

The fungus in a lichen is which of the following?

A. a basidiomycete
B. an ascomycete
C. a zygomycete
D. an apicomplexan

B

Short Answer

What are three ways that lichens are environmentally valuable?

Contributor

  • Nina Parker, (Shenandoah University), Mark Schneegurt (Wichita State University), Anh-Hue Thi Tu (Georgia Southwestern State University), Philip Lister (Central New Mexico Community College), and Brian M. Forster (Saint Joseph’s University) with many contributing authors. Original content via Openstax (CC BY 4.0; Access for free at https://openstax.org/books/microbiology/pages/1-introduction)


Current

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Volume 5 - 2014 - Index

9. Traditional use of Gymnopus nubicola as food resource in a Kichwa community, Pichincha, Ecuador
Gamboa-Trujillo JP, Wartchow F, Cerón C, Aules E, Aigaje C, Calvalcanti LH, Gibertoni TB
Mycosphere 5(1), 180–186, Doi 10.5943/mycosphere/5/1/9

15. Three new species of Mycena sect. Longisetae
Aravindakshan DM, Manimohan P
Mycosphere 5(2), 290–297, Doi 10.5943/mycosphere/5/2/3

17. A new species of Fusticeps from Thailand
Chuaseeharonnachai C, Somrithipol S, Boonyuen N
Mycosphere 5(2), 313–317, Doi 10.5943/mycosphere/5/2/5

21. Pyrenophora
Ariyawansa HA, Kang JC, Alias SA, Chukeatirote E, Hyde KD
Mycosphere 5(2), 351–362, Doi 10.5943/mycosphere/5/2/9

29. Two new records of Cyathus species for South America
Barbosa MMB, Cruz RHSF, Calonge FD, Baseia IG
Mycosphere 5(3), 425–428, Doi 10.5943/mycosphere/5/3/5

32. A new species of Berkleasmium from Ulyanovsk, Russia
Hüseyin E, Selçuk F, Churakov BP
Mycosphere 5(3), 462-466, Doi 10.5943/mycosphere/5/3/8

35. Additions to the myxobiota of Central America
Rojas C, Calvo E
Mycosphere 5(3), 488–495, Doi 10.5943/mycosphere/5/3/11

36. The genus Blumenavia (Clathraceae, Phallales)
Trierveiler-Pereira L, Alves CR, Silveira RMB
Mycosphere 5(3), 496-501, Doi 10.5943/mycosphere/5/3/12

39. A new combination in Phytopythium: P. kandeliae (Oomycetes, Straminipila)
Marano AV, Jesus AL, de Souza JI, Leaño EM, James TY, Jerônimo GH, de Cock AWAM, Pires-Zottarelli CLA
Mycosphere 5(4), 510-522, Doi 10.5943/mycosphere/5/4/3

44. New species of Diderma from Vietnam
Novozhilov YK, Mitchell DW, Okun MV, Shchepin ON
Mycosphere 5(4), 554–564, Doi 10.5943/mycosphere/5/4/8

45. Growth and light emission of luminous basidiomycetes cultivated on solid media and in submerged culture
Medvedeva SE, Artemenko KS, Krivosheenko AA, Rusinova AG, Rodicheva EK, Puzyr AP, Bondar VS
Mycosphere 5(4), 565–577, Doi 10.5943/mycosphere/5/4/9

46. Diversity studies on the endophytic fungi of Vitex negundo L.
Sunayana N, Nalini MS, Sampath Kumara KK, Prakash HS
Mycosphere 5(4), 578–590, Doi 10.5943/mycosphere/5/4/10

47. Two new species and new records of hyphomycetes from Vietnam
Mel&rsquonik VA, Alexandrova AV, Braun U
Mycosphere 5(4), 591–600, Doi 10.5943/mycosphere/5/4/11

50. Two new species of Russula (Russulales) from India
Das K, Dowie NJ, Li GJ, Miller SL
Mycosphere 5(5), 612–622, Doi 10.5943/mycosphere/5/5/2

51. The genus Pythiogeton (Pythiogetonaceae) in Brazil
Rocha JRS, Sousa NDC, Santos LA, Pereira AA, Negreiros NC, Sales PCL, Trindade-Júnior OC
Mycosphere 5(5), 623–634, Doi 10.5943/mycosphere/5/5/3

54. Munkovalsaria donacina from grapevines and Desert Ash in Australia
Pitt WM, Úrbez-Torres JR, Trouillas FP
Mycosphere 5(5), 656–661, Doi 10.5943/mycosphere/5/5/6

55. Myxomycetes from three lowland tropical forests in Vietnam
Tran DQ, Nguyen HTN, Tran HTM, Stephenson SL
Mycosphere 5(5), 662–672, Doi 10.5943/mycosphere/5/5/7

57. Lichen as indicator of metal pollution in the vicinity of SIPCOT industries in Cuddalore, southeast coast of India
Logesh AR, Upadhyay AK, Joshi S, Kalaiselvam M, Upreti DK, Shukla AC
Mycosphere 5(5), 681–688, Doi 10.5943/mycosphere/5/5/9

58. Cystoderma castellanum, a new species from Spain
Blanco-Dios JB
Mycosphere 5(5), 688–691, Doi 10.5943/mycosphere/5/5/10

59. Does habitat loss affect tropical myxomycetes?
Rojas C, Doss RG
Mycosphere 5(5), 692–700, Doi 10.5943/mycosphere/5/5/11

61. First survey for protosteloid amoebae in South Australia
Tice AK, Fry NW, Stephenson SL
Mycosphere 5(6), 706–710, Doi 10.5943/mycosphere/5/6/1

63. Rare lichen taxa from Costa Rica
Neuwirth G, Stocker-Wörgötter E
Mycosphere 5(6), 711–718, Doi 10.5943/mycosphere/5/6/2

66. A new method by correlation to forecast the optimal time of spore-prints production and collection on sporocarps of Ganoderma resinaceum Boud
Kengni Ayissi MB, Mossebo DC, Machouart M, Kansci G, Tsigaing TF, Dogang LR, Metsebing BP, Difack NM
Mycosphere 5(6), 758–767, Doi 10.5943/mycosphere/5/6/6

67. On the status of Phytopythium kandeliae (Oomycetes, Straminipila)
Marano AV, Jesus AL, de Souza JI, Jerônimo GH, Pires-Zottarelli CLA
Mycosphere 5(6), 768–769, Doi 10.5943/mycosphere/5/6/7

69. The genera Ascobolus and Saccobolus (Ascobolaceae, Pezizales) in Brazil
Melo RFR, Miller AN, Santiago ALCMA, Maia LC
Mycosphere 5(6), 790–804, DRoger Melo oi 10.5943/mycosphere/5/6/9

71. First cultivation of Agaricus flocculosipes and a novel Thai strain of A. subrufescens
Thongklang N, Sysouphanthong P, Callac P, Hyde KD
Mycosphere 5(6), 814–820, Doi 10.5943/mycosphere/5/6/11

72. The response of myxogastrids to soil amendments
Feest A, Stephenson SL
Mycosphere 5(6), 821–829, Doi 10.5943/mycosphere/5/6/12

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Contents

In American English, "lichen" is pronounced the same as the verb "liken" ( / ˈ l aɪ k ən / ). In British English, both this pronunciation and one rhyming with "kitchen" / ˈ l ɪ tʃ ən / ) are used. [17] [18] [19]

English lichen derives from Greek λειχήν leichēn ("tree moss, lichen, lichen-like eruption on skin") via Latin lichen. [20] [21] [22] The Greek noun, which literally means "licker", derives from the verb λείχειν leichein, "to lick". [23] [24]

Like the word moss, the word lichen is also used as an uncountable noun, as in "Lichen grows on rocks".

Lichens grow in a wide range of shapes and forms (morphologies). The shape of a lichen is usually determined by the organization of the fungal filaments. [25] The nonreproductive tissues, or vegetative body parts, are called the thallus. Lichens are grouped by thallus type, since the thallus is usually the most visually prominent part of the lichen. Thallus growth forms typically correspond to a few basic internal structure types. Common names for lichens often come from a growth form or color that is typical of a lichen genus.

Common groupings of lichen thallus growth forms are:

    [26][27][28] – growing like a tuft or multiple-branched leafless mini-shrub, upright or hanging down, 3-dimensional branches with nearly round cross section (terete) or flattened [26][27] – growing in 2-dimensional, flat, leaf-like lobes [5][26][27] – crust-like, adhering tightly to a surface (substrate) like a thick coat of paint [28] – formed of small leaf-like scales crustose below but free at the tips [29] – powdery – jelly-like – stringy or like matted hair – wispy, like teased wool
  1. structureless

There are variations in growth types in a single lichen species, grey areas between the growth type descriptions, and overlapping between growth types, so some authors might describe lichens using different growth type descriptions.

When a crustose lichen gets old, the center may start to crack up like old-dried paint, old-broken asphalt paving, or like the polygonal "islands" of cracked-up mud in a dried lakebed. This is called being rimose or areolate, and the "island" pieces separated by the cracks are called areolas. [26] The areolas appear separated, but are (or were) [ citation needed ] connected by an underlying "prothallus" or "hypothallus". [29] When a crustose lichen grows from a center and appears to radiate out, it is called crustose placodioid. When the edges of the areolas lift up from the substrate, it is called squamulose. [30] : 159 [28]

These growth form groups are not precisely defined. Foliose lichens may sometimes branch and appear to be fruticose. Fruticose lichens may have flattened branching parts and appear leafy. Squamulose lichens may appear where the edges lift up. Gelatinous lichens may appear leafy when dry. [30] : 159 Means of telling them apart in these cases are in the sections below.

Structures involved in reproduction often appear as discs, bumps, or squiggly lines on the surface of the thallus. [7] : 4 The thallus is not always the part of the lichen that is most visually noticeable. Some lichens can grow inside solid rock between the grains (endolithic lichens), with only the sexual fruiting part visible growing outside the rock. [26] These may be dramatic in color or appearance. [26] Forms of these sexual parts are not in the above growth form categories. [26] The most visually noticeable reproductive parts are often circular, raised, plate-like or disc-like outgrowths, with crinkly edges, and are described in sections below.

Color Edit

Lichens come in many colors. [7] : 4 Coloration is usually determined by the photosynthetic component. [25] Special pigments, such as yellow usnic acid, give lichens a variety of colors, including reds, oranges, yellows, and browns, especially in exposed, dry habitats. [31] In the absence of special pigments, lichens are usually bright green to olive gray when wet, gray or grayish-green to brown when dry. [31] This is because moisture causes the surface skin (cortex) to become more transparent, exposing the green photobiont layer. [31] Different colored lichens covering large areas of exposed rock surfaces, or lichens covering or hanging from bark can be a spectacular display when the patches of diverse colors "come to life" or "glow" in brilliant displays following rain.

Different colored lichens may inhabit different adjacent sections of a rock face, depending on the angle of exposure to light. [31] Colonies of lichens may be spectacular in appearance, dominating much of the surface of the visual landscape in forests and natural places, such as the vertical "paint" covering the vast rock faces of Yosemite National Park. [32]

Color is used in identification. [33] : 4 The color of a lichen changes depending on whether the lichen is wet or dry. [33] Color descriptions used for identification are based on the color that shows when the lichen is dry. [33] Dry lichens with a cyanobacterium as the photosynthetic partner tend to be dark grey, brown, or black. [33]

The underside of the leaf-like lobes of foliose lichens is a different color from the top side (dorsiventral), often brown or black, sometimes white. A fruticose lichen may have flattened "branches", appearing similar to a foliose lichen, but the underside of a leaf-like structure on a fruticose lichen is the same color as the top side. The leaf-like lobes of a foliose lichen may branch, giving the appearance of a fruticose lichen, but the underside will be a different color from the top side. [29]

The sheen on some jelly-like gelatinous lichens is created by mucilaginous secretions. [25]

Internal structure and growth forms Edit

A lichen consists of a simple photosynthesizing organism, usually a green alga or cyanobacterium, surrounded by filaments of a fungus. Generally, most of a lichen's bulk is made of interwoven fungal filaments, [34] although in filamentous and gelatinous lichens [25] this is not the case. The fungus is called a mycobiont. The photosynthesizing organism is called a photobiont. Algal photobionts are called phycobionts. [35] Cyanobacteria photobionts are called cyanobionts. [35]

The part of a lichen that is not involved in reproduction, the "body" or "vegetative tissue" of a lichen, is called the thallus. The thallus form is very different from any form where the fungus or alga are growing separately. The thallus is made up of filaments of the fungus called hyphae. The filaments grow by branching then rejoining to create a mesh, which is called being "anastomose". The mesh of fungal filaments may be dense or loose.

Generally, the fungal mesh surrounds the algal or cyanobacterial cells, often enclosing them within complex fungal tissues that are unique to lichen associations. The thallus may or may not have a protective "skin" of densely packed fungal filaments, often containing a second fungal species, [1] which is called a cortex. Fruticose lichens have one cortex layer wrapping around the "branches". Foliose lichens have an upper cortex on the top side of the "leaf", and a separate lower cortex on the bottom side. Crustose and squamulose lichens have only an upper cortex, with the "inside" of the lichen in direct contact with the surface they grow on (the substrate). Even if the edges peel up from the substrate and appear flat and leaf-like, they lack a lower cortex, unlike foliose lichens. Filamentous, byssoid, leprose, [29] gelatinous, and other lichens do not have a cortex, which is called being ecorticate. [36]

Fruticose, foliose, crustose, and squamulose lichens generally have up to three different types of tissue, differentiated by having different densities of fungal filaments. [34] The top layer, where the lichen contacts the environment, is called a cortex. [34] The cortex is made of densely tightly woven, packed, and glued together (agglutinated) fungal filaments. [34] The dense packing makes the cortex act like a protective "skin", keeping other organisms out, and reducing the intensity of sunlight on the layers below. [34] The cortex layer can be up to several hundred micrometers (μm) in thickness (less than a millimeter). [37] The cortex may be further topped by an epicortex of secretions, not cells, 0.6–1 μm thick in some lichens. [37] This secretion layer may or may not have pores. [37]

Below the cortex layer is a layer called the photobiontic layer or symbiont layer. [27] [34] The symbiont layer has less densely packed fungal filaments, with the photosynthetic partner embedded in them. [34] The less dense packing allows air circulation during photosynthesis, similar to the anatomy of a leaf. [34] Each cell or group of cells of the photobiont is usually individually wrapped by hyphae, and in some cases penetrated by a haustorium. [25] In crustose and foliose lichens, algae in the photobiontic layer are diffuse among the fungal filaments, decreasing in gradation into the layer below. In fruticose lichens, the photobiontic layer is sharply distinct from the layer below. [25]

The layer beneath the symbiont layer called is called the medulla. The medulla is less densely packed with fungal filaments than the layers above. In foliose lichens, there is usually, as in Peltigera, [30] : 159 another densely packed layer of fungal filaments called the lower cortex. [29] [34] Root-like fungal structures called rhizines (usually) [30] : 159 grow from the lower cortex to attach or anchor the lichen to the substrate. [2] [29] Fruticose lichens have a single cortex wrapping all the way around the "stems" and "branches". [30] The medulla is the lowest layer, and may form a cottony white inner core for the branchlike thallus, or it may be hollow. [30] : 159 Crustose and squamulose lichens lack a lower cortex, and the medulla is in direct contact with the substrate that the lichen grows on.

In crustose areolate lichens, the edges of the areolas peel up from the substrate and appear leafy. In squamulose lichens the part of the lichen thallus that is not attached to the substrate may also appear leafy. But these leafy parts lack a lower cortex, which distinguishes crustose and squamulose lichens from foliose lichens. [34] Conversely, foliose lichens may appear flattened against the substrate like a crustose lichen, but most of the leaf-like lobes can be lifted up from the substrate because it is separated from it by a tightly packed lower cortex. [29]

Gelatinous, [30] : 159 byssoid, and leprose lichens lack a cortex (are ecorticate), and generally have only undifferentiated tissue, similar to only having a symbiont layer. [ citation needed ]

In lichens that include both green algal and cyanobacterial symbionts, the cyanobacteria may be held on the upper or lower surface in small pustules called cephalodia.

Pruinia is a whitish coating on top of an upper surface. [38] An epinecral layer is "a layer of horny dead fungal hyphae with indistinct lumina in or near the cortex above the algal layer". [38]

In August 2016, it was reported that macrolichens have more than one species of fungus in their tissues. [1]

Symbiotic relation Edit

Lichens are fungi that have discovered agriculture

A lichen is a composite organism that emerges from algae or cyanobacteria living among the filaments (hyphae) of the fungi in a mutually beneficial symbiotic relationship. The fungi benefit from the carbohydrates produced by the algae or cyanobacteria via photosynthesis. The algae or cyanobacteria benefit by being protected from the environment by the filaments of the fungi, which also gather moisture and nutrients from the environment, and (usually) provide an anchor to it. Although some photosynthetic partners in a lichen can survive outside the lichen, the lichen symbiotic association extends the ecological range of both partners, whereby most descriptions of lichen associations describe them as symbiotic. Both partners gain water and mineral nutrients mainly from the atmosphere, through rain and dust. The fungal partner protects the alga by retaining water, serving as a larger capture area for mineral nutrients and, in some cases, provides minerals obtained from the substrate. If a cyanobacterium is present, as a primary partner or another symbiont in addition to a green alga as in certain tripartite lichens, they can fix atmospheric nitrogen, complementing the activities of the green alga.

In three different lineages the fungal partner has independently lost the mitochondrial gene atp9, which has key functions in mitochondrial energy production. The loss makes the fungi completely dependent on their symbionts. [40]

The algal or cyanobacterial cells are photosynthetic and, as in plants, they reduce atmospheric carbon dioxide into organic carbon sugars to feed both symbionts. Phycobionts (algae) produce sugar alcohols (ribitol, sorbitol, and erythritol), which are absorbed by the mycobiont (fungus). [35] Cyanobionts produce glucose. [35] Lichenized fungal cells can make the photobiont "leak" out the products of photosynthesis, where they can then be absorbed by the fungus. [7] : 5

It appears many, probably the majority, of lichen also live in a symbiotic relationship with an order of basidiomycete yeasts called Cyphobasidiales. The absence of this third partner could explain the difficulties of growing lichen in the laboratory. The yeast cells are responsible for the formation of the characteristic cortex of the lichen thallus, and could also be important for its shape. [41]

The lichen combination of alga or cyanobacterium with a fungus has a very different form (morphology), physiology, and biochemistry than the component fungus, alga, or cyanobacterium growing by itself, naturally or in culture. The body (thallus) of most lichens is different from those of either the fungus or alga growing separately. When grown in the laboratory in the absence of its photobiont, a lichen fungus develops as a structureless, undifferentiated mass of fungal filaments (hyphae). If combined with its photobiont under appropriate conditions, its characteristic form associated with the photobiont emerges, in the process called morphogenesis. [4] In a few remarkable cases, a single lichen fungus can develop into two very different lichen forms when associating with either a green algal or a cyanobacterial symbiont. Quite naturally, these alternative forms were at first considered to be different species, until they were found growing in a conjoined manner.

Evidence that lichens are examples of successful symbiosis is the fact that lichens can be found in almost every habitat and geographic area on the planet. [12] Two species in two genera of green algae are found in over 35% of all lichens, but can only rarely be found living on their own outside of a lichen. [42]

In a case where one fungal partner simultaneously had two green algae partners that outperform each other in different climates, this might indicate having more than one photosynthetic partner at the same time might enable the lichen to exist in a wider range of habitats and geographic locations. [12]

At least one form of lichen, the North American beard-like lichens, are constituted of not two but three symbiotic partners: an ascomycetous fungus, a photosynthetic alga, and, unexpectedly, a basidiomycetous yeast. [43]

Phycobionts can have a net output of sugars with only water vapor. [35] The thallus must be saturated with liquid water for cyanobionts to photosynthesize. [35]

Algae produce sugars that are absorbed by the fungus by diffusion into special fungal hyphae called appressoria or haustoria in contact with the wall of the algal cells. [44] The appressoria or haustoria may produce a substance that increases permeability of the algal cell walls, and may penetrate the walls. [44] The algae may contribute up to 80% of their sugar production to the fungus. [44]

Ecology Edit

Lichen associations may be examples of mutualism or commensalism, but the lichen relationship can be considered parasitic [45] under circumstances where the photosynthetic partner can exist in nature independently of the fungal partner, but not vice versa. Photobiont cells are routinely destroyed in the course of nutrient exchange. The association continues because reproduction of the photobiont cells matches the rate at which they are destroyed. [45] The fungus surrounds the algal cells, [8] often enclosing them within complex fungal tissues unique to lichen associations. In many species the fungus penetrates the algal cell wall, [8] forming penetration pegs (haustoria) similar to those produced by pathogenic fungi that feed on a host. [28] [46] Cyanobacteria in laboratory settings can grow faster when they are alone rather than when they are part of a lichen.

Miniature ecosystem and holobiont theory Edit

Symbiosis in lichens is so well-balanced that lichens have been considered to be relatively self-contained miniature ecosystems in and of themselves. [12] [13] It is thought that lichens may be even more complex symbiotic systems that include non-photosynthetic bacterial communities performing other functions as partners in a holobiont. [14] [15]

Many lichens are very sensitive to environmental disturbances and can be used to cheaply [8] assess air pollution, [47] [48] [49] ozone depletion, and metal contamination. Lichens have been used in making dyes, perfumes, [50] and in traditional medicines. A few lichen species are eaten by insects [8] or larger animals, such as reindeer. [51] Lichens are widely used as environmental indicators or bio-indicators. When air is very badly polluted with sulphur dioxide, there may be no lichens present only some green algae can tolerate those conditions. If the air is clean, then shrubby, hairy and leafy lichens become abundant. A few lichen species can tolerate fairly high levels of pollution, and are commonly found in urban areas, on pavements, walls and tree bark. The most sensitive lichens are shrubby and leafy, while the most tolerant lichens are all crusty in appearance. Since industrialisation, many of the shrubby and leafy lichens such as Ramalina, Usnea and Lobaria species have very limited ranges, often being confined to the areas which have the cleanest air.

Lichenicolous fungi Edit

Some fungi can only be found living on lichens as obligate parasites. These are referred to as lichenicolous fungi, and are a different species from the fungus living inside the lichen thus they are not considered to be part of the lichen. [52]

Reaction to water Edit

Moisture makes the cortex become more transparent. [7] : 4 This way, the algae can conduct photosynthesis when moisture is available, and is protected at other times. When the cortex is more transparent, the algae show more clearly and the lichen looks greener.

Metabolites, metabolite structures and bioactivity Edit

Lichens can show intense antioxidant activity. [53] [54] Secondary metabolites are often deposited as crystals in the apoplast. [55] Secondary metabolites are thought to play a role in preference for some substrates over others. [55]

Sometimes lichens contain structures made from fungal metabolites, for example crustose lichens sometimes have a polysaccharide [ clarification needed ] layer in the cortex. [ citation needed ]

Growth rate Edit

Lichens often have a regular but very slow growth rate of less than a millimeter per year.

In crustose lichens, the area along the margin is where the most active growth is taking place. [30] : 159 Most crustose lichens grow only 1–2 mm in diameter per year.

Life span Edit

Lichens may be long-lived, with some considered to be among the oldest living organisms. [4] [16] Lifespan is difficult to measure because what defines the "same" individual lichen is not precise. [56] Lichens grow by vegetatively breaking off a piece, which may or may not be defined as the "same" lichen, and two lichens can merge, then becoming the "same" lichen. [56] An Arctic species called "map lichen" (Rhizocarpon geographicum) has been dated at 8,600 years, apparently the world's oldest living organism. [57]

Response to environmental stress Edit

Unlike simple dehydration in plants and animals, lichens may experience a complete loss of body water in dry periods. [8] Lichens are capable of surviving extremely low levels of water content (poikilohydric). [58] : 5–6 They quickly absorb water when it becomes available again, becoming soft and fleshy. [8] Reconfiguration of membranes following a period of dehydration requires several minutes or more. [ citation needed ]

In tests, lichen survived and showed remarkable results on the adaptation capacity of photosynthetic activity within the simulation time of 34 days under Martian conditions in the Mars Simulation Laboratory (MSL) maintained by the German Aerospace Center (DLR). [59] [60]

The European Space Agency has discovered that lichens can survive unprotected in space. In an experiment led by Leopoldo Sancho from the Complutense University of Madrid, two species of lichen—Rhizocarpon geographicum and Xanthoria elegans—were sealed in a capsule and launched on a Russian Soyuz rocket 31 May 2005. Once in orbit, the capsules were opened and the lichens were directly exposed to the vacuum of space with its widely fluctuating temperatures and cosmic radiation. After 15 days, the lichens were brought back to earth and were found to be unchanged in their ability to photosynthesize. [61] [62]

Vegetative reproduction Edit

Many lichens reproduce asexually, either by a piece breaking off and growing on its own (vegetative reproduction) or through the dispersal of diaspores containing a few algal cells surrounded by fungal cells. [2] Because of the relative lack of differentiation in the thallus, the line between diaspore formation and vegetative reproduction is often blurred. Fruticose lichens can easily [ citation needed ] fragment, and new lichens can grow from the fragment (vegetative reproduction). [ citation needed ] Many lichens break up into fragments when they dry, dispersing themselves by wind action, to resume growth when moisture returns. [63] [64] Soredia (singular: "soredium") are small groups of algal cells surrounded by fungal filaments that form in structures called soralia, from which the soredia can be dispersed by wind. [2] Isidia (singular: "isidium") are branched, spiny, elongated, outgrowths from the thallus that break off for mechanical dispersal. [2] Lichen propagules (diaspores) typically contain cells from both partners, although the fungal components of so-called "fringe species" rely instead on algal cells dispersed by the "core species". [65]

Sexual reproduction Edit

Structures involved in reproduction often appear as discs, bumps, or squiggly lines on the surface of the thallus. [7] : 4 Though it has been argued that sexual reproduction in photobiont is selected against, there are strong evidence that suggest meiotic activities (sexual reproduction) in Trebouxia. [66] [67] Many lichen fungi reproduce sexually like other fungi, producing spores formed by meiosis and fusion of gametes. Following dispersal, such fungal spores must meet with a compatible algal partner before a functional lichen can form.

Some lichen fungi belong to Basidiomycetes (basidiolichens) and produce mushroom-like reproductive structures resembling those of their nonlichenized relatives.

Most lichen fungi belong to Ascomycetes (ascolichens). Among the ascolichens, spores are produced in spore-producing structures called ascomata. [7] The most common types of ascomata are the apothecium (plural: apothecia) and perithecium (plural: perithecia). [7] : 14 Apothecia are usually cups or plate-like discs located on the top surface of the lichen thallus. When apothecia are shaped like squiggly line segments instead of like discs, they are called lirellae. [7] : 14 Perithecia are shaped like flasks that are immersed in the lichen thallus tissue, which has a small hole for the spores to escape the flask, and appear like black dots on the lichen surface. [7] : 14

The three most common spore body types are raised discs called apothecia (singular: apothecium), bottle-like cups with a small hole at the top called perithecia (singular: perithecium), and pycnidia (singular: pycnidium), shaped like perithecia but without asci (an ascus is the structure that contains and releases the sexual spores in fungi of the Ascomycota). [68]

The apothecium has a layer of exposed spore-producing cells called asci (singular: ascus), and is usually a different color from the thallus tissue. [7] : 14 When the apothecium has an outer margin, the margin is called the exciple. [7] : 14 When the exciple has a color similar to colored thallus tissue the apothecium or lichen is called lecanorine, meaning similar to members of the genus Lecanora. [7] : 14 When the exciple is blackened like carbon it is called lecideine meaning similar to members of the genus Lecidea. [7] : 14 When the margin is pale or colorless it is called biatorine. [7] : 14

A "podetium" (plural: podetia) is a lichenized stalk-like structure of the fruiting body rising from the thallus, associated with some fungi that produce a fungal apothecium. [27] Since it is part of the reproductive tissue, podetia are not considered part of the main body (thallus), but may be visually prominent. [27] The podetium may be branched, and sometimes cup-like. They usually bear the fungal pycnidia or apothecia or both. [27] Many lichens have apothecia that are visible to the naked eye. [2]

Most lichens produce abundant sexual structures. [69] Many species appear to disperse only by sexual spores. [69] For example, the crustose lichens Graphis scripta and Ochrolechia parella produce no symbiotic vegetative propagules. Instead, the lichen-forming fungi of these species reproduce sexually by self-fertilization (i.e. they are homothallic). This breeding system may enable successful reproduction in harsh environments. [69]

Mazaedia (singular: mazaedium) are apothecia shaped like a dressmaker's pin in (pin lichen)s, where the fruiting body is a brown or black mass of loose ascospores enclosed by a cup-shaped exciple, which sits on top of a tiny stalk. [7] : 15

Lichens are classified by the fungal component. Lichen species are given the same scientific name (binomial name) as the fungus species in the lichen. Lichens are being integrated into the classification schemes for fungi. The alga bears its own scientific name, which bears no relationship to that of the lichen or fungus. [70] There are about 13,500–17,000 identified lichen species. [44] Nearly 20% of known fungal species are associated with lichens. [44]

"Lichenized fungus" may refer to the entire lichen, or to just the fungus. This may cause confusion without context. A particular fungus species may form lichens with different algae species, giving rise to what appear to be different lichen species, but which are still classified (as of 2014) as the same lichen species. [71]

Formerly, some lichen taxonomists placed lichens in their own division, the Mycophycophyta, but this practice is no longer accepted because the components belong to separate lineages. Neither the ascolichens nor the basidiolichens form monophyletic lineages in their respective fungal phyla, but they do form several major solely or primarily lichen-forming groups within each phylum. [72] Even more unusual than basidiolichens is the fungus Geosiphon pyriforme, a member of the Glomeromycota that is unique in that it encloses a cyanobacterial symbiont inside its cells. Geosiphon is not usually considered to be a lichen, and its peculiar symbiosis was not recognized for many years. The genus is more closely allied to endomycorrhizal genera. Fungi from Verrucariales also form marine lichens with the brown algae Petroderma maculiforme, [73] and have a symbiotic relationship with seaweed like (rockweed) and Blidingia minima, where the algae are the dominant components. The fungi is thought to help the rockweeds to resist desiccation when exposed to air. [74] [75] In addition, lichens can also use yellow-green algae (Heterococcus) as their symbiotic partner. [76]

Lichens independently emerged from fungi associating with algae and cyanobacteria multiple times throughout history. [77]

Fungi Edit

The fungal component of a lichen is called the mycobiont. The mycobiont may be an Ascomycete or Basidiomycete. [10] The associated lichens are called either ascolichens or basidiolichens, respectively. Living as a symbiont in a lichen appears to be a successful way for a fungus to derive essential nutrients, since about 20% of all fungal species have acquired this mode of life. [78]

Thalli produced by a given fungal symbiont with its differing partners may be similar, [ citation needed ] and the secondary metabolites identical, [ citation needed ] indicating [ citation needed ] that the fungus has the dominant role in determining the morphology of the lichen. But the same mycobiont with different photobionts may also produce very different growth forms. [71] Lichens are known in which there is one fungus associated with two or even three algal species.

Although each lichen thallus generally appears homogeneous, some evidence seems to suggest that the fungal component may consist of more than one genetic individual of that species. [ citation needed ]

Two or more fungal species can interact to form the same lichen. [79]

The following table lists the orders and families of fungi that include lichen-forming species.

Photobionts Edit

The photosynthetic partner in a lichen is called a photobiont. The photobionts in lichens come from a variety of simple prokaryotic and eukaryotic organisms. In the majority of lichens the photobiont is a green alga (Chlorophyta) or a cyanobacterium. In some lichens both types are present. Algal photobionts are called phycobionts, while cyanobacterial photobionts are called cyanobionts. [35] According to one source, about 90% of all known lichens have phycobionts, and about 10% have cyanobionts, [35] while another source states that two thirds of lichens have green algae as phycobiont, and about one third have a cyanobiont. [28] Approximately 100 species of photosynthetic partners from 40 [35] genera and five distinct classes (prokaryotic: Cyanophyceae eukaryotic: Trebouxiophyceae, Phaeophyceae, Chlorophyceae) have been found to associate with the lichen-forming fungi. [80]

Common algal photobionts are from the genera Trebouxia, Trentepohlia, Pseudotrebouxia, or Myrmecia. Trebouxia is the most common genus of green algae in lichens, occurring in about 40% of all lichens. "Trebouxioid" means either a photobiont that is in the genus Trebouxia, or resembles a member of that genus, and is therefore presumably a member of the class Trebouxiophyceae. [27] The second most commonly represented green alga genus is Trentepohlia. [28] Overall, about 100 species of eukaryotes are known to occur as photobionts in lichens. All the algae are probably able to exist independently in nature as well as in the lichen. [79]

A "cyanolichen" is a lichen with a cyanobacterium as its main photosynthetic component (photobiont). [81] Most cyanolichen are also ascolichens, but a few basidiolichen like Dictyonema and Acantholichen have cyanobacteria as their partner. [82]

The most commonly occurring cyanobacterium genus is Nostoc. [79] Other [28] common cyanobacterium photobionts are from Scytonema. [10] Many cyanolichens are small and black, and have limestone as the substrate. [ citation needed ] Another cyanolichen group, the jelly lichens of the genera Collema or Leptogium are gelatinous and live on moist soils. Another group of large and foliose species including Peltigera, Lobaria, and Degelia are grey-blue, especially when dampened or wet. Many of these characterize the Lobarion communities of higher rainfall areas in western Britain, e.g., in the Celtic rain forest. Strains of cyanobacteria found in various cyanolichens are often closely related to one another. [83] They differ from the most closely related free-living strains. [83]

The lichen association is a close symbiosis. It extends the ecological range of both partners but is not always obligatory for their growth and reproduction in natural environments, since many of the algal symbionts can live independently. A prominent example is the alga Trentepohlia, which forms orange-coloured populations on tree trunks and suitable rock faces. Lichen propagules (diaspores) typically contain cells from both partners, although the fungal components of so-called "fringe species" rely instead on algal cells dispersed by the "core species". [65]

The same cyanobiont species can occur in association with different fungal species as lichen partners. [84] The same phycobiont species can occur in association with different fungal species as lichen partners. [35] More than one phycobiont may be present in a single thallus. [35]

A single lichen may contain several algal genotypes. [85] [86] These multiple genotypes may better enable response to adaptation to environmental changes, and enable the lichen to inhabit a wider range of environments. [87]

Controversy over classification method and species names Edit

There are about 20,000 known lichen species. [10] But what is meant by "species" is different from what is meant by biological species in plants, animals, or fungi, where being the same species implies that there is a common ancestral lineage. [10] Because lichens are combinations of members of two or even three different biological kingdoms, these components must have a different ancestral lineage from each other. By convention, lichens are still called "species" anyway, and are classified according to the species of their fungus, not the species of the algae or cyanobacteria. Lichens are given the same scientific name (binomial name) as the fungus in them, which may cause some confusion. The alga bears its own scientific name, which has no relationship to the name of the lichen or fungus. [70]

Depending on context, "lichenized fungus" may refer to the entire lichen, or to the fungus when it is in the lichen, which can be grown in culture in isolation from the algae or cyanobacteria. Some algae and cyanobacteria are found naturally living outside of the lichen. The fungal, algal, or cyanobacterial component of a lichen can be grown by itself in culture. When growing by themselves, the fungus, algae, or cyanobacteria have very different properties than those of the lichen. Lichen properties such as growth form, physiology, and biochemistry, are very different from the combination of the properties of the fungus and the algae or cyanobacteria.

The same fungus growing in combination with different algae or cyanobacteria, can produce lichens that are very different in most properties, meeting non-DNA criteria for being different "species". Historically, these different combinations were classified as different species. When the fungus is identified as being the same using modern DNA methods, these apparently different species get reclassified as the same species under the current (2014) convention for classification by fungal component. This has led to debate about this classification convention. These apparently different "species" have their own independent evolutionary history. [2] [71]

There is also debate as to the appropriateness of giving the same binomial name to the fungus, and to the lichen that combines that fungus with an alga or cyanobacterium (synecdoche). This is especially the case when combining the same fungus with different algae or cyanobacteria produces dramatically different lichen organisms, which would be considered different species by any measure other than the DNA of the fungal component. If the whole lichen produced by the same fungus growing in association with different algae or cyanobacteria, were to be classified as different "species", the number of "lichen species" would be greater.

Diversity Edit

The largest number of lichenized fungi occur in the Ascomycota, with about 40% of species forming such an association. [70] Some of these lichenized fungi occur in orders with nonlichenized fungi that live as saprotrophs or plant parasites (for example, the Leotiales, Dothideales, and Pezizales). Other lichen fungi occur in only five orders in which all members are engaged in this habit (Orders Graphidales, Gyalectales, Peltigerales, Pertusariales, and Teloschistales). Overall, about 98% of lichens have an ascomycetous mycobiont. [88] Next to the Ascomycota, the largest number of lichenized fungi occur in the unassigned fungi imperfecti, a catch-all category for fungi whose sexual form of reproduction has never been observed. [ citation needed ] Comparatively few Basidiomycetes are lichenized, but these include agarics, such as species of Lichenomphalia, clavarioid fungi, such as species of Multiclavula, and corticioid fungi, such as species of Dictyonema.

Identification methods Edit

Lichen identification uses growth form and reactions to chemical tests.

The outcome of the "Pd test" is called "Pd", which is also used as an abbreviation for the chemical used in the test, para-phenylenediamine. [27] If putting a drop on a lichen turns an area bright yellow to orange, this helps identify it as belonging to either the genus Cladonia or Lecanora. [27]

Evolution and paleontology Edit

The fossil record for lichens is poor. [89] The extreme habitats that lichens dominate, such as tundra, mountains, and deserts, are not ordinarily conducive to producing fossils. [89] [90] There are fossilized lichens embedded in amber. The fossilized Anzia is found in pieces of amber in northern Europe and dates back approximately 40 million years. [91] Lichen fragments are also found in fossil leaf beds, such as Lobaria from Trinity County in northern California, USA, dating back to the early to middle Miocene. [92]

The oldest fossil lichen in which both symbiotic partners have been recovered is Winfrenatia, an early zygomycetous (Glomeromycotan) lichen symbiosis that may have involved controlled parasitism, [ citation needed ] is permineralized in the Rhynie Chert of Scotland, dating from early Early Devonian, about 400 million years ago. [93] The slightly older fossil Spongiophyton has also been interpreted as a lichen on morphological [94] and isotopic [95] grounds, although the isotopic basis is decidedly shaky. [96] It has been demonstrated that Silurian-Devonian fossils Nematothallus [97] and Prototaxites [98] were lichenized. Thus lichenized Ascomycota and Basidiomycota were a component of Early Silurian-Devonian terrestrial ecosystems. [99] [100] Newer research suggests that lichen evolved after the evolution of land plants. [101]

The ancestral ecological state of both Ascomycota and Basidiomycota was probably saprobism, and independent lichenization events may have occurred multiple times. [102] [103] In 1995, Gargas and colleagues proposed that there were at least five independent origins of lichenization three in the basidiomycetes and at least two in the Ascomycetes. [104] However, Lutzoni et al. (2001) indicate that lichenization probably evolved earlier and was followed by multiple independent losses. Some non-lichen-forming fungi may have secondarily lost the ability to form a lichen association. As a result, lichenization has been viewed as a highly successful nutritional strategy. [105] [106]

Lichenized Glomeromycota may extend well back into the Precambrian. Lichen-like fossils consisting of coccoid cells (cyanobacteria?) and thin filaments (mucoromycotinan Glomeromycota?) are permineralized in marine phosphorite of the Doushantuo Formation in southern China. These fossils are thought to be 551 to 635 million years old or Ediacaran. [107] Ediacaran acritarchs also have many similarities with Glomeromycotan vesicles and spores. [108] It has also been claimed that Ediacaran fossils including Dickinsonia, [109] were lichens, [110] although this claim is controversial. [111] Endosymbiotic Glomeromycota comparable with living Geosiphon may extend back into the Proterozoic in the form of 1500 million year old Horodyskia [112] and 2200 million year old Diskagma. [113] Discovery of these fossils suggest that fungi developed symbiotic partnerships with photoautotrophs long before the evolution of vascular plants, though the Ediacaran lichen hypothesis is largely rejected due to an inappropriate definition of lichens based on taphonomy and substrate ecology. [114]

Substrates and habitats Edit

Lichens cover about 7% of the planet's surface and grow on and in a wide range of substrates and habitats, including some of the most extreme conditions on earth. [115] They are abundant growing on bark, leaves, and hanging from branches "living on thin air" (epiphytes) in rain forests and in temperate woodland. They grow on bare rock, walls, gravestones, roofs, and exposed soil surfaces. They can survive in some of the most extreme environments on Earth: arctic tundra, hot dry deserts, rocky coasts, and toxic slag heaps. They can live inside solid rock, growing between the grains, and in the soil as part of a biological soil crust in arid habitats such as deserts. Some lichens do not grow on anything, living out their lives blowing about the environment. [2]

When growing on mineral surfaces, some lichens slowly decompose their substrate by chemically degrading and physically disrupting the minerals, contributing to the process of weathering by which rocks are gradually turned into soil. While this contribution to weathering is usually benign, it can cause problems for artificial stone structures. For example, there is an ongoing lichen growth problem on Mount Rushmore National Memorial that requires the employment of mountain-climbing conservators to clean the monument.

Lichens are not parasites on the plants they grow on, but only use them as a substrate to grow on. The fungi of some lichen species may "take over" the algae of other lichen species. [8] [116] Lichens make their own food from their photosynthetic parts and by absorbing minerals from the environment. [8] Lichens growing on leaves may have the appearance of being parasites on the leaves, but they are not. However, some lichens, notably those of the genus Diploschistes are known to parasitise other lichens. Diploschistes muscorum starts its development in the tissue of a host Cladonia species. [46] : 30 [28] : 171

In the arctic tundra, lichens, together with mosses and liverworts, make up the majority of the ground cover, which helps insulate the ground and may provide forage for grazing animals. An example is "Reindeer moss", which is a lichen, not a moss. [8]

A crustose lichen that grows on rock is called a saxicolous lichen. [27] [30] : 159 Crustose lichens that grow on the rock are epilithic, and those that grow immersed inside rock, growing between the crystals with only their fruiting bodies exposed to the air, are called endolithic lichens. [26] [30] : 159 [81] A crustose lichen that grows on bark is called a corticolous lichen. [30] : 159 A lichen that grows on wood from which the bark has been stripped is called a lignicolous lichen. [36] Lichens that grow immersed inside plant tissues are called endophloidic lichens or endophloidal lichens. [26] [30] : 159 Lichens that use leaves as substrates, whether the leaf is still on the tree or on the ground, are called epiphyllous or foliicolous. [35] A terricolous lichen grows on the soil as a substrate. Many squamulous lichens are terricolous. [30] : 159 Umbillicate lichens are foliose lichens that are attached to the substrate at only one point. [26] A vagrant lichen is not attached to a substrate at all, and lives its life being blown around by the wind.

Lichens and soils Edit

In addition to distinct physical mechanisms by which lichens break down raw stone, recent studies indicate lichens attack stone chemically, entering newly chelated minerals into the ecology.

The lichen exudates, which have powerful chelating capacity, the widespread occurrence of mineral neoformation, particularly metal oxalates, together with the characteristics of weathered substrates, all confirm the significance of lichens as chemical weathering agents. [117]

Over time, this activity creates new fertile soil from lifeless stone.

Lichens may be important in contributing nitrogen to soils in some deserts through being eaten, along with their rock substrate, by snails, which then defecate, putting the nitrogen into the soils. [118] Lichens help bind and stabilize soil sand in dunes. [2] In deserts and semi-arid areas, lichens are part of extensive, living biological soil crusts, essential for maintaining the soil structure. [2] Lichens have a long fossil record in soils dating back 2.2 billion years. [113]

Ecological interactions Edit

Lichens are pioneer species, among the first living things to grow on bare rock or areas denuded of life by a disaster. [2] Lichens may have to compete with plants for access to sunlight, but because of their small size and slow growth, they thrive in places where higher plants have difficulty growing. Lichens are often the first to settle in places lacking soil, constituting the sole vegetation in some extreme environments such as those found at high mountain elevations and at high latitudes. [119] Some survive in the tough conditions of deserts, and others on frozen soil of the Arctic regions. [120]

A major ecophysiological advantage of lichens is that they are poikilohydric (poikilo- variable, hydric- relating to water), meaning that though they have little control over the status of their hydration, they can tolerate irregular and extended periods of severe desiccation. Like some mosses, liverworts, ferns, and a few "resurrection plants", upon desiccation, lichens enter a metabolic suspension or stasis (known as cryptobiosis) in which the cells of the lichen symbionts are dehydrated to a degree that halts most biochemical activity. In this cryptobiotic state, lichens can survive wider extremes of temperature, radiation and drought in the harsh environments they often inhabit.

Lichens do not have roots and do not need to tap continuous reservoirs of water like most higher plants, thus they can grow in locations impossible for most plants, such as bare rock, sterile soil or sand, and various artificial structures such as walls, roofs and monuments. Many lichens also grow as epiphytes (epi- on the surface, phyte- plant) on plants, particularly on the trunks and branches of trees. When growing on plants, lichens are not parasites they do not consume any part of the plant nor poison it. Lichens produce allelopathic chemicals that inhibit the growth of mosses. Some ground-dwelling lichens, such as members of the subgenus Cladina (reindeer lichens), produce allelopathic chemicals that leach into the soil and inhibit the germination of seeds, spruce and other plants. [121] Stability (that is, longevity) of their substrate is a major factor of lichen habitats. Most lichens grow on stable rock surfaces or the bark of old trees, but many others grow on soil and sand. In these latter cases, lichens are often an important part of soil stabilization indeed, in some desert ecosystems, vascular (higher) plant seeds cannot become established except in places where lichen crusts stabilize the sand and help retain water.

Lichens may be eaten by some animals, such as reindeer, living in arctic regions. The larvae of a number of Lepidoptera species feed exclusively on lichens. These include common footman and marbled beauty. However, lichens are very low in protein and high in carbohydrates, making them unsuitable for some animals. Lichens are also used by the Northern Flying Squirrel for nesting, food, and a water source during winter.

Effects of air pollution Edit

If lichens are exposed to air pollutants at all times, without any deciduous parts, they are unable to avoid the accumulation of pollutants. Also lacking stomata and a cuticle, lichens may absorb aerosols and gases over the entire thallus surface from which they may readily diffuse to the photobiont layer. [122] Because lichens do not possess roots, their primary source of most elements is the air, and therefore elemental levels in lichens often reflect the accumulated composition of ambient air. The processes by which atmospheric deposition occurs include fog and dew, gaseous absorption, and dry deposition. [123] Consequently, many environmental studies with lichens emphasize their feasibility as effective biomonitors of atmospheric quality. [122] [124] [125] [126] [127] [128]

Not all lichens are equally sensitive to air pollutants, so different lichen species show different levels of sensitivity to specific atmospheric pollutants. [129] The sensitivity of a lichen to air pollution is directly related to the energy needs of the mycobiont, so that the stronger the dependency of the mycobiont on the photobiont, the more sensitive the lichen is to air pollution. [130] Upon exposure to air pollution, the photobiont may use metabolic energy for repair of its cellular structures that would otherwise be used for maintenance of its photosynthetic activity, therefore leaving less metabolic energy available for the mycobiont. The alteration of the balance between the photobiont and mycobiont can lead to the breakdown of the symbiotic association. Therefore, lichen decline may result not only from the accumulation of toxic substances, but also from altered nutrient supplies that favor one symbiont over the other. [122]

This interaction between lichens and air pollution has been used as a means of monitoring air quality since 1859, with more systematic methods developed by William Nylander in 1866. [2]

Food Edit

Lichens are eaten by many different cultures across the world. Although some lichens are only eaten in times of famine, others are a staple food or even a delicacy. Two obstacles are often encountered when eating lichens: lichen polysaccharides are generally indigestible to humans, and lichens usually contain mildly toxic secondary compounds that should be removed before eating. Very few lichens are poisonous, but those high in vulpinic acid or usnic acid are toxic. [131] Most poisonous lichens are yellow.

In the past, Iceland moss (Cetraria islandica) was an important source of food for humans in northern Europe, and was cooked as a bread, porridge, pudding, soup, or salad. Bryoria fremontii was an important food in parts of North America, where it was usually pitcooked. Northern peoples in North America and Siberia traditionally eat the partially digested reindeer lichen (Cladina spp.) after they remove it from the rumen of caribou or reindeer that have been killed. Rock tripe (Umbilicaria spp. and Lasalia spp.) is a lichen that has frequently been used as an emergency food in North America, and one species, Umbilicaria esculenta, is used in a variety of traditional Korean and Japanese foods.

Lichenometry Edit

Lichenometry is a technique used to determine the age of exposed rock surfaces based on the size of lichen thalli. Introduced by Beschel in the 1950s, [132] the technique has found many applications. it is used in archaeology, palaeontology, and geomorphology. It uses the presumed regular but slow rate of lichen growth to determine the age of exposed rock. [32] : 9 [133] Measuring the diameter (or other size measurement) of the largest lichen of a species on a rock surface indicates the length of time since the rock surface was first exposed. Lichen can be preserved on old rock faces for up to [ citation needed ] 10,000 years, providing the maximum age limit of the technique, though it is most accurate (within 10% error) when applied to surfaces that have been exposed for less than 1,000 years. [134] Lichenometry is especially useful for dating surfaces less than 500 years old, as radiocarbon dating techniques are less accurate over this period. [135] The lichens most commonly used for lichenometry are those of the genera Rhizocarpon (e.g. the species Rhizocarpon geographicum) and Xanthoria.

Biodegradation Edit

Lichens have been shown to degrade polyester resins, as can be seen in archaeological sites in the Roman city of Baelo Claudia in Spain. [136] Lichens can accumulate several environmental pollutants such as lead, copper, and radionuclides. [137] Some species of lichen such as Parmelia sulcata and Lobaria pulmonaria, and many in the Cladonia genus have been shown to produce serine proteases capable of the degradation of pathogenic forms of prion protein (PrP), which may be useful in treating contaminated environmental reservoirs. [138] [139] [140]

As dyes Edit

Many lichens produce secondary compounds, including pigments that reduce harmful amounts of sunlight and powerful toxins that reduce herbivory or kill bacteria. These compounds are very useful for lichen identification, and have had economic importance as dyes such as cudbear or primitive antibiotics.

The pH indicator (indicated acidic or basic) in the litmus test is a dye extracted from the lichen Roccella tinctoria by boiling.

In the Highlands of Scotland, traditional dyes for Harris tweed [2] and other traditional cloths were made from lichens, including the orange Xanthoria parietina and the grey foliaceous Parmelia saxatilis common on rocks known as "crottle".

There are reports dating almost 2,000 years old of lichens being used to make purple and red dyes. [141] Of great historical and commercial significance are lichens belonging to the family Roccellaceae, commonly called orchella weed or orchil. Orcein and other lichen dyes have largely been replaced by synthetic versions.

Traditional medicine and research Edit

Historically in traditional medicine of Europe, Lobaria pulmonaria was collected in large quantities as "Lungwort", due to its lung-like appearance (the doctrine of signatures suggesting that herbs can treat body parts that they physically resemble). Similarly, Peltigera leucophlebia was used as a supposed cure for thrush, due to the resemblance of its cephalodia to the appearance of the disease. [28]

Lichens produce metabolites in research for their potential therapeutic or diagnostic value. [142] Some metabolites produced by lichens are structurally and functionally similar to broad-spectrum antibiotics while few are associated respectively to antiseptic similarities. [143] Usnic acid is the most commonly studied metabolite produced by lichens. [143] It is also under research as an bactericidal agent against Escherichia coli and Staphylococcus aureus. [144]

Aesthetic appeal Edit

Colonies of lichens may be spectacular in appearance, dominating the surface of the visual landscape as part of the aesthetic appeal to visitors of Yosemite National Park, Sequoia National Park and the Bay of Fires. [32] : 2 Orange and yellow lichens add to the ambience of desert trees, rock faces, tundras, and rocky seashores. Intricate webs of lichens hanging from tree branches add a mysterious aspect to forests. Fruticose lichens are used in model railroading [145] and other modeling hobbies as a material for making miniature trees and shrubs.

In literature Edit

In early Midrashic literature, the Hebrew word "vayilafeth" in Ruth 3:8 is explained as referring to Ruth entwining herself around Boaz like lichen. [146] The tenth century Arab physician, Al-Tamimi, mentions lichens dissolved in vinegar and rose water being used in his day for the treatment of skin diseases and rashes. [147]

The plot of John Wyndham's novel Trouble with Lichen revolves around an anti-aging chemical extracted from a lichen.

Although lichens had been recognized as organisms for quite some time, it was not until 1867, when Swiss botanist Simon Schwendener proposed his dual theory of lichens, that lichens are a combination of fungi with algae or cyanobacteria, whereby the true nature of the lichen association began to emerge. [148] Schwendener's hypothesis, which at the time lacked experimental evidence, arose from his extensive analysis of the anatomy and development in lichens, algae, and fungi using a light microscope. Many of the leading lichenologists at the time, such as James Crombie and Nylander, rejected Schwendener's hypothesis because the common consensus was that all living organisms were autonomous. [148]

Other prominent biologists, such as Heinrich Anton de Bary, Albert Bernhard Frank, Melchior Treub and Hermann Hellriegel were not so quick to reject Schwendener's ideas and the concept soon spread into other areas of study, such as microbial, plant, animal and human pathogens. [148] [149] When the complex relationships between pathogenic microorganisms and their hosts were finally identified, Schwendener's hypothesis began to gain popularity. Further experimental proof of the dual nature of lichens was obtained when Eugen Thomas published his results in 1939 on the first successful re-synthesis experiment. [148]

In the 2010s, a new facet of the fungi-algae partnership was discovered. Toby Spribille and colleagues found that many types of lichen that were long thought to be ascomycete-algae pairs were actually ascomycete-basidiomycete-algae trios. [1] [150]

Lobaria pulmonaria, tree lungwort, lung lichen, lung moss Upper Bavaria, Germany

Cladonia macilenta var. bacillaris 'Lipstick Cladonia'

Usnea australis, a fruticose form, growing on a tree branch

Hypogymnia cf. tubulosa with Bryoria sp. and Tuckermannopsis sp. in the Canadian Rockies

Letharia sp. with Bryoria sp. on pine branches near Blackpine Lake, Washington State

Lobaria oregana, commonly called 'Lettuce lichen', in the Hoh Rainforest, Washington State

A lichen growing on a rock in a Brazilian cloud forest

Xanthoparmelia cf. lavicola, a foliose lichen, on basalt.

Physcia millegrana (a foliose lichen), with an unlichenized polypore fungus (bottom right), on a fallen log.

Crustose lichens on limestone in Alta Murgia-Southern Italy

Cladonia cf. cristatella, a lichen commonly referred to as 'British Soldiers'. Notice the red tips.

Foliose lichens on rock growing outward and dying in the center. These lichens are at least several decades old.

Xanthoria sp. lichen on volcanic rock in Craters of the Moon National Monument (Idaho, USA)

Lecanora cf. muralis lichen on the banks of the Bega canal in Timișoara, Romania

Microscopic view of lichen growing on a piece of concrete dust. [note 1]


In vitro antitumor activities of the lichen compounds olivetoric, physodic and psoromic acid in rat neuron and glioblastoma cells

Context Since methods utilised in the treatment of glioblastoma multiforme (GBM) are inadequate and have too many side effects, usage of herbal products in the treatment process comes into prominence. Lichens are symbiotic organisms used for medicinal purposes for many years. There are various anticancer treatments about components of two lichen species used in the present study. Objective Antitumor potential of three lichen secondary metabolites including olivetoric acid (OLA) and physodic acid (PHA) isolated from Pseudevernia furfuracea (L.) Zopf (Parmeliaceae) and psoromic acid (PSA) isolated from Rhizoplaca melanophthalma (DC.) Leuckert (Lecanoraceae) were investigated on human U87MG-GBM cell lines and primary rat cerebral cortex (PRCC) cells for the first time. Materials and methods PRCC cells used as healthy brain cells were obtained from Sprague-Dawley rats. The treatments were carried out on the cells cultured for 48 h. Cytotoxic effects of different concentrations (2.5, 5, 10, 20 and 40 mg/L) of metabolites on the cells were determined via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) analyses. Total antioxidant capacity (TAC) and total oxidant status (TOS) parameters were used for assessing oxidative alterations. Oxidative DNA damage potentials of metabolites were investigated via evaluating 8-hydroxy-2'-deoxyguanosine (8-OH-dG) levels. Results Median inhibitory concentration (IC50) values of OLA, PHA and PSA were 125.71, 698.19 and 79.40 mg/L for PRCC cells and 17.55, 410.72 and 56.22 mg/L for U87MG cells, respectively. It was revealed that cytotoxic effects of these metabolites showed positive correlation with concentration, LDH activity and oxidative DNA damage. Discussion and conclusion The present findings obtained in this study revealed that primarily OLA and then PSA had high potential for use in the treatment of GBM.

Keywords: Cytotoxicity genotoxicity glioblastoma multiforme oxidative status secondary metabolite.


5.5: Lichens - Biology

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Lichen-based indices to quantify responses to climate and air pollution across northeastern U.S.A

Susan Will-Wolf, 1,* Sarah Jovan, 2 Peter Neitlich, 3 JeriLynn E. Peck, 4 Roger Rosentreter 5

1 1 Department of Botany, University of Wisconsin-Madison, 430 Lincoln Drive, Madison, WI 53706, U.S.A
2 2 USDA Forest Service, Portland Forestry Sciences Lab, 620 SW Main, Suite 400, Portland, OR 97205, U
3 3 Western Arctic National Parklands, National Park Service, 41A Wandling Road, Winthrop, WA 98862, U
4 4 Department of Ecosystem Science & Management, The Pennsylvania State University, 207 Forest Resour
5 5 Biology Department, Boise State University, Boise, ID 83642, U.S.A.

* Corresponding author’s e-mail: [email protected]

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Lichens are known to be indicators for air quality they also respond to climate. We developed indices for lichen response to climate and air quality in forests across the northeastern United States of America (U.S.A.), using 218–250 plot surveys with 145–161 macrolichen taxa from the Forest Inventory and Analysis (FIA) Program of the U.S. Department of Agriculture, Forest Service. Lichen indicator species for response to climate and air quality were selected using Indicator Species Analysis, correlations with environmental variables, and published literature. Ordinations were used to evaluate the strength and relationships of the final indices. The Pollution Index was calculated for a plot from abundances of 12 tolerant and 45 sensitive indicator species standardized by abundance of all lichen species. The Index was correlated with modeled deposition of acidifying sulfur and oxidized nitrogen and with lichen community ordination pollution axes. Analyses suggested separate response of lichens to fertilizing N (weak statistical support). The Climate Index, from abundances of 19 warmer and 47 cooler climate indicator species, was correlated with modeled minimum January and annual maximum temperatures, and with ordination climate axes. The two indices are statistically independent. Repeat sample variability for each index was 7–14.5% (lower with higher quality data), supporting detection of consistent trends of 16–20% change over time or variation across space. Variability of the Climate Index was more affected by data quality than that of the Pollution Index. The continuous gradient of Pollution Index values suggests the cleanest areas may have air pollution above a critical load to fully protect lichen communities. These Indices can be applied to track lichen responses using other data from our study regions suitability should be tested before use outside of the study area.

The American Bryological and Lichenological Society, Inc.

Susan Will-Wolf , Sarah Jovan , Peter Neitlich , JeriLynn E. Peck , and Roger Rosentreter "Lichen-based indices to quantify responses to climate and air pollution across northeastern U.S.A," The Bryologist 118(1), 59-82, (24 March 2015). https://doi.org/10.1639/0007-2745-118.1.059

Received: 29 December 2014 Accepted: 1 February 2015 Published: 24 March 2015


7. Find and identify five lichens, one liverwort, and six mosses.

There are thousands of species of lichens, liverworts, and mosses, and providing an identification guide is well beyond the scope of this chapter. In order to meet this requirement, you will most likely need to obtain a field guide. Here are a few possibilities:

Another alternative is to find an online key:

The best approach for this requirement is to familiarize yourself with these plants as much as you can, and then go outside and see what's there. Whenever you come across a specimen, try to identify it. If possible, collect a bit of it and take it home for a closer examination. This is far better than deciding that you are going to go out and find Cladonia portentosa. In other words, instead of deciding what you're going to find and then going out to look for it, go out and see what's there and then identify it.


Potential and pitfalls of eukaryotic metagenome skimming: a test case for lichens

Whole-genome shotgun sequencing of multispecies communities using only a single library layout is commonly used to assess taxonomic and functional diversity of microbial assemblages. Here, we investigate to what extent such metagenome skimming approaches are applicable for in-depth genomic characterizations of eukaryotic communities, for example lichens. We address how to best assemble a particular eukaryotic metagenome skimming data, what pitfalls can occur, and what genome quality can be expected from these data. To facilitate a project-specific benchmarking, we introduce the concept of twin sets, simulated data resembling the outcome of a particular metagenome sequencing study. We show that the quality of genome reconstructions depends essentially on assembler choice. Individual tools, including the metagenome assemblers Omega and MetaVelvet, are surprisingly sensitive to low and uneven coverages. In combination with the routine of assembly parameter choice to optimize the assembly N50 size, these tools can preclude an entire genome from the assembly. In contrast, MIRA, an all-purpose overlap assembler, and SPAdes, a multisized de Bruijn graph assembler, facilitate a comprehensive view on the individual genomes across a wide range of coverage ratios. Testing assemblers on a real-world metagenome skimming data from the lichen Lasallia pustulata demonstrates the applicability of twin sets for guiding method selection. Furthermore, it reveals that the assembly outcome for the photobiont Trebouxia sp. falls behind the a priori expectation given the simulations. Although the underlying reasons remain still unclear, this highlights that further studies on this organism require special attention during sequence data generation and downstream analysis.

Fig. S3. k mer coverage plot of the 0:10 twin set for k = 141.

Fig. S4. k mer coverage plot of the 1:9 twin set for k = 131.

Fig. S5. k mer coverage plot of the 2:8 twin set for k = 131.

Fig. S6. k mer coverage plot of the 3:7 twin set for k = 141.

Fig. S7. k mer coverage plot of the 4:6 twin set for k = 141.

Fig. S8. k mer coverage plot of the 5:5 twin set for k = 91.

Fig. S9. k mer coverage plot of the 6:4 twin set for k = 131.

Fig. S10. k mer coverage plot of the 7:3 twin set for k = 51.

Fig. S11. k mer coverage plot of the 8:2 twin set for k = 51.

Fig. S12. k mer coverage plot of the 9:1 twin set for k = 151.

Fig. S13. k mer coverage plot of the 10:0 twin set for k = 131.

Table S1. Quast report of the 0:10 set.

Table S2. Quast report of the 1:9 set.

Table S3. Quast report of the 2:8 set.

Table S4. Quast report of the 3:7 set.

Table S5. Quast report of the 4:6 set.

Table S6. Quast report of the 5:5 set.

Table S7. Quast report of the 6:4 set.

Table S8. Quast report of the 7:3 set.

Table S9. Quast report of the 8:2 set.

Table S10. Quast report of the 9:1 set.

Table S11. Quast report of the 10:0 set.

Table S12. Quast report of the Lasallia pustulata metagenomic data set.

Table S13. Quast report of the fungal contigs of the Lasallia pustulata data set.

Table S14. Quast report of the algal contigs of the Lasallia pustulata data set.

Table S15. Quast report of the bacterial contigs of the Lasallia pustulata data set.

Table S16. Quast report of the 10× coverage algal data.

Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.


Contents

Several members of the family Sphaerophoraceae

Pilophorus acicularis (Cladoniaceae)

The species was first described in 1803 as Baeomyces acicularis by the Swedish botanist and "father of lichenology" Erik Acharius. [5] The taxon was transferred to several different genera in the next few decades resulting in several synonyms, including Cenomyces acicularis (by Acharius in 1810), Cladonia acicularis (Elias Magnus Fries in 1831), and Stereocaulon aciculare (Edward Tuckerman in 1845). [1] Elias Fries's son Thore Magnus transferred the species to his then newly created genus Pilophorus in 1857. [6] William Nylander also published the combination Pilophorus acicularis in 1857, [7] but later analysis suggested that Fries's combination was published first, and under the Principle of Priority, the correct citation of the species is Pilophorus acicularis (Ach.) Th.Fr. (1857). [8]

The genus Pilophorus was until recently considered to be a member of the family Stereocaulaceae by some authors. [9] [10] [11] Analysis of small subunit ribosomal DNA sequences showed P. acicularis to be more closely related to the Cladoniaceae, rather than the Stereocaulaceae. [12]

The specific epithet aciculare is derived from the Latin acicularis, meaning "needle-like". [13] The lichen is commonly known as the "devil's matchstick" [14] the common name for the genus—"nail lichen"—is also used. [15]

The thallus is the vegetative body of a lichen that contains the lichen mycobiont (fungus) and the photobiont (algae and/or cyanobacteria). In P. acicularis, the primary thallus (thallus horizontalis) is spread out like a granular crust on the surface of its substrate. It is light green when young, but becomes gray in age or when dry. [16] The pseudopodetia (upright stalk-like extensions of the thallus made of vegetative tissue) range from 0.5 to 3 cm (0.2 to 1.2 in) high, and are about 1 mm thick they grow in dense clusters. Most pseudopodetia are either unbranched or forked into two branches, with the stalks curved so as to appear as if combed less frequently, they are erect like pins, and up to 1 cm (0.4 in) tall. Some specimens are highly branched in the upper part of the pseudopodetia, causing them to bear some resemblance to P. robustus, although this morphology is uncommon. Internally, the pseudopodetia are solid when young, becoming hollow with age, and are composed of long, thin, highly gelatinized hyphae with narrow cavities about 0.5 μm wide. The lower part of older pseudopodetia becomes blackened internally. The algal layer is not continuous—contrasting with lichen species that have thalli that stratify into discrete tissue types, including a photobiont layer—and occurs with the mycobiont in the form of granules. These granules may be absent from some parts of the thallus surface. Pycnidia (flask-like structures, resembling perithecia, in which conidia are produced) occur in the tips of small sterile pseudopodetia or in the tips of small lateral branches of older pseudopodetia. [9]

The conidiophores of P. acicularis are 30 μm long, and unbranched. They have terminal sickle-shaped conidia that measure 6 by 1 μm. The apothecia (reproductive structures covered with the spore-producing asci) are abundant, usually with one or several on the tips of the pseudopodetia. They are black, hemispherical or roughly triangular, and measure up to 1.5 mm in diameter. The hymenium (the fertile spore-bearing layer of cells containing the asci) is up to 240 μm thick, and about two-thirds of it is pigmented the lower part of the hymenium is sterile, consisting of only paraphyses. The asci are eight-spored. The ascospores are rounded when young, becoming spindle-shaped when mature, with dimensions of 21.0–29.5 by 4.5–5.5 μm. The generative tissue (hyphae that eventually forms the thallus) is closely interwoven with short, broad cells that have large cavities. The generative tissue is pigmented black-brown, with the color being most intense below the paraphyses, becoming less so towards the stalk region. [9]

Pilophorus acicularis is a tripartite lichen—containing a fungus, a green alga, and a cyanobacterium. Cephalodia (lichenized aggregations of nitrogen-fixing cyanobacteria) are present on the primary thallus smaller cephalodia are also on the pseudopodetia. Hemispherical to irregularly shaped, and light to dark brown in color, they contain species from the genus Nostoc. [8] The green algal photosynthetic symbiont (photobiont) associated with P. acicularis is Asterochloris magna (formerly Trebouxia magna). [17] [18]

Similar species Edit

Pilophorus acicularis can be separated from similar species by its tall pseudopodetia. It may be confused with P. robustus, especially in material from Alaska where both species occur together. Usually, the different branching (umbellate in P. robustus versus dichotomous in P. acicularis) and the lack of a columella (an internal, column-shaped structure) in longitudinal sections of the pseudopodetia of P. acicularis make it relatively easy to distinguish between the two. [9]

Pilophyllus clavatus, a species found in Western North America, Japan, Taiwan and South Korea, resembles P. acicularis, but it has much shorter pseudopodetia—up to 1.5 cm (0.6 in) long. [19]

The lichen typically grows on silicate stone, rarely on decaying wood. [9] It is usually in partial shade in openings in low to mid-elevation moist forests, and is also frequently found in rocky roadcuts. [16] Lichens with cephalodia are capable of fixing nitrogen, and contribute nitrogen to the ecosystem. [20]

P. acicularis is probably the most abundant species of the genus. Most specimens have been found on the west coast of North America as far north as Alaska, [21] but it has been reported most frequently from British Columbia and Washington. The species is found in China, [22] Japan, [23] Korea, [24] and Taiwan, [25] and has also been reported from the Russian arctic. [26] In general, P. acicularis seems to prefer an oceanic climate without extremely low temperatures, at least in comparison with other species of the genus. This assumption is supported by the fact that P. acicularis is found more southerly (34 findings in California) than all other species and is less frequently found in northern Alaska where, for example, P. robustus and P. vegae are more common. P. acicularis is rare east of the Rocky Mountains. [9]


Journal of Environmental Biology

Due to climate change, occurrence of both higher and lower plants in forest areas is deteriorated considerably. Keeping this in view, a survey was undertaken to study the distribution of lichen communities in Yercaud hills belonging to Eastern Ghats, Tamil Nadu, India. The results indicated that distribution of lichens was registered from 700 m above msl in silver oak trees and the same was noted from 900 m above msl in rock surface. Distribution of lichen colonies was abundant, while temperature was low along with sunshine and RH was high along with rainfall pattern.? Sunshine was negatively correlated with lichen distribution, but rainfall was positively correlated. These biologically important hills exhibited a total of 61 different lichen species covering 13 families with 33 genera in the survey. In addition, about 22 new species of lichens were recorded in the lichen database of Yercaud hills. The results revealed that lichens grew well in the pH range of 4.3 - 5.5 in silver oak tree barks and 4.5 - 6.0 in rocks, which coincided with the buffering action of bark and rock. There was a direct relationship between the moisture content of tree barks and rocks and lichen adherence. Estimation of various micro and macro elements including ash contents were found to be more in fruticose than in foliose lichen and lest in crustose lichens. The study revealed that there was a drastic reduction in lichen density due to low tree bark moisture content and pH values owing to change in environmental parameters at Yercaud hills.?

Eastern Ghats, Environment, Lichen, Survey of lichens, Yercaud hills

Copyright ? 2016 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).


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